nextflex-96 dna barcodes  (Bioo Scientific)

Journal: Microbiology Spectrum

Article Title: Library Preparation and Sequencing Platform Introduce Bias in Metagenomic-Based Characterizations of Microbiomes

doi: 10.1128/spectrum.00090-22

Figure Lengend Snippet: Study design and comparison between sample groups. (A) Two pig feces samples and two sewage samples were processed directly or after storage at −80°C for 64 h. The DNA isolation was performed in duplicates, respectively. Library preparation and sequencing were performed in four different combinations, NEXTflex PCR-Free library preparation with sequencing on a HiSeq (NEXTflex HiSeq), KAPA PCR-free library preparation with sequencing on a HiSeq (KAPA HiSeq), NEXTflex PCR-Free library preparation with sequencing on a NextSeq (NEXTflex NextSeq), and Nextera library preparation with sequencing on a NextSeq (Nextera NextSeq). The latter sequencing strategy was performed twice (Nextera 1 NextSeq and Nextera 2 NextSeq). The setup resulted in a total of 80 metagenomes plus 5 negative controls (i.e., DNA extraction controls). (B) Boxplots display pairwise Aitchison distances between different groupings of samples. Within the different groups, dots representing the distances were colored according to which sample the comparison was made in. Blue dots represent a distance between two different samples.

Article Snippet: The NEXTflex library preparation was run PCR free with NEXTflex barcodes (NEXTflex-96 DNA barcodes) according to the manufacturer’s recommendations (Bioo Scientific NEXTflex PCR-Free DNA sequencing kit; catalog no. 5142-01).

Techniques: DNA Extraction, Sequencing